UCSDのYunde ZHAO先生のSpecial Seminarのご紹介です．

会場：東京大学農学部（弥生キャンパス）2号館化学第2講義室121

日時：3月17日（日）16:45-17:45*

*当日，会場の東京大学農学部では，日本育種学会の講演会が行われており，この特別セミナーは第5会場で通常講演終了後に

「グループ研究集会（大会参加者以外も参加可能）」として行われます．

奮ってご参加ください．

On improving gene editing technology for auxin research and crop improvement

Yunde Zhao  

Department of Cell and Developmental Biology, University of California San Diego, La Jolla, CA 92093-0116, USA  

Gene editing has revolutionized both basic plant biological research and crop improvement. My group has developed several technologies that enable speedy and precise modifications of genes in plants. Our technologies including Ribozyme-based CRIPSR, Transgene Killer CRISPR, and the RUBY reporter gene have been widely used in both plants and animals. The technological advances provide unprecedented tools for us to study auxin biology, a main focus of our research program. In this presentation, I will first describe our recent unpublished progress in Homology-Directed Repair, which enables us to in situ tag genes with green fluorescent protein and other epitopes. Our technologies avoid the pitfalls of transgenic approaches such as co-suppression and ectopic overexpression. The technologies have led to surprising insights into several well characterized auxin genes and a discovery of new genetic interaction model called “haplo-complementation”.